CD138+ Plasma Cell Enrichment from Whole Blood and Bone Marrow

CD138 expression is a hallmark of plasma cells and multiple myeloma cells, and it is considered a useful marker in plasma cells tumors diagnostics. Using our newly developed MARS® CS system (multi-physics automated reconfigurable separation) we demonstrate a cost-efficient, fast and robust workflow for isolation of CD138+ cells from whole blood and bone marrow samples within a short time. We achieved 64.5% purity with a recovery higher than 90% of CD138+ cells isolated from a blood sample, and 59% purity of cells isolated from U266-spiked bone marrow sample.