Stem cell isolation
Harnessing Innovation
Transforming CD34+ Cell Isolation
Multipotent hematopoietic stem cell (HSC) therapy has become increasingly popular due to its wide range of health benefits and research potential.3 The CD34 protein is considered the primary marker of HSCs and hematopoietic progenitor cells, as it is expressed on the majority of these cells.4 The current process used to isolate CD34+ HSCs is time-consuming and typically results in considerable loss of cells. The MARS® platform uses a more efficient method, eliminating the need for red blood cell (RBC) lysis and density gradient centrifugation, while yielding high purity samples.
3. Lee JY, Hong SH. Hematopoietic Stem Cells and Their Roles in Tissue Regeneration. Int J Stem Cells. 2020 Mar 30;13(1):1-12.
4. Ivanovic Z. Hematopoietic stem cells in research and clinical applications: The “CD34 issue”. World J Stem Cells. 2010 Apr 26;2(2):18-23.
MARS Platform: Shifting Barriers to Breakthroughs
Discover the breakthrough capabilities of the MARS® platform. Our column-free in-flow technology ensures not just unparalleled flexibility in workflow optimization, but also maximizes purity and recovery rates. You can directly isolate target cells—even those at low percentages—from samples including PBMC, peripheral blood, CBMC, Cord blood or bone marrow. Dive into a world of precision and efficiency
AUTOMATED PRECISION
automatic 2x or 3x separation guarantees unmatched consistency and a seamless user experience
UNRIVALED RECOVERY
AND PURITY
far superior to conventional methods, our technology ensures high cell purity and recovery that consistently outperforms
EFFICIENT, ECONOMICAL,
AND REUSABLE
reusable and cleanable fluidics dramatically reduces the cost, while pre-set cleaning protocols offer unprecedented efficiency
Consistent Excellence across Variety of Samples
CD34+ Cell Isolation From Mobilized Blood
Mobilized blood is often used as a source of CD34+ cells and stands as a cornerstone for breakthroughs in HSC research and therapeutic innovations. CD34+ cells not only pave the way for deeper insights into hematopoiesis and hematological disorders but also play a crucial role in regenerative medicine. The MARS® platform steps up to the challenge, offering a cost-effective and efficient process that optimizes the purity, viability, and yield of CD34+ cells.
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CD34+ Cell Isolation
From Cord Blood Mononuclear Cells
Isolation of CD34+ cells is key for HSC research and therapeutic advancements as these cells may enhance the understanding of hematopoiesis, hematological disorders, and regenerative medicine. This application note summarizes the efficient and cost-saving process employed by the MARS® platform, which enhances the purity, viability, and yield of CD34+ cells.
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Cost-Effective, Time-Efficient
Discover a transformative approach to cell separation with the MARS® platform. Not only can you reduce assay costs by an impressive 60%, but you can also cut experiment time by nearly 50% for a 50 mL input sample. Elevate your workflow and efficiency instantly!
*The processing time and estimated cost information provided by our user is intended for general guidance only. We do not guarantee its accuracy, completeness, or suitability for any particular purpose. We disclaim any responsibility or liability for any decisions made or actions taken based on this information.
Faster, Simpler, Better
Experience an unparalleled ease in cell separation with the MARS® platform. Cut down both overall and hands-on time by nearly an hour, and follow a seamless workflow: from labeling protocol straight to two (or optionally three) MARS® Immunomagnetic isolation runs. Dive into efficiency today!
Current workflow
example for 100M cells isolation
CORD BLOOD
MNC ISOLATION BY FICOLL DENSITY GRADIENT CENTRIFUGATION
50 min
RBC LYSIS
10 min
2x CENTRIFUGATION
20 min
ADD FCR BLOCKER
1 min
INCUBATE WITH ANTI CD34 MICROBEADS
30 min
CENTRIFUGATION
10 min
MANUAL COLUMN 3x WASH
20 min
PLUNGE
1 min
ISOLATED CD34+ CELLS
TOTAL: 142 min
MARS workflow
example for 100M cells isolation
CORD BLOOD
MNC ISOLATION BY FICOLL DENSITY GRADIENT CENTRIFUGATION
50 min
INCUBATE WITH ANTI CD34 COCKTAIL
15 min
INCUBATE WITH MAGNETIC BEADS
10 min
RUN A SERIAL PROGRAM
20 min
ISOLATED CD34+ CELLS
TOTAL: 95 min
Automated, Simplified Isolation Process
Delve into the MARS® platform’s seamless cell isolation experience. Our intuitive workflow guarantees high purity and superior cell recovery rates with minimal hands-on time. Automated one- to three-pass cell enrichment ensures efficiency at every step, together with our matrix-free isolation, cost-effective consumables, and reusable fluidics. Plus, swiftly re-run samples through the magnetic channel, amplifying your operational efficiency. Dive into a world where innovation meets simplicity.
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Cell Therapy
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