Mass Cytometry

Comprehensive solution for sample preparation

Efficient Sample Preparation for CyTOF

Mass cytometry, referred to as cytometry by Time-Of-Flight (CyTOF®), is a powerful technology for high-dimensional and high-throughput single-cell assays. CyTOF has been broadly used across applications including immunophenotyping for hematopoietic stem cell transplantation, tumor microenvironment, and cancer immunotherapy.1

CyTOF allows for analysis of up to 60 parameters simultaneously. This capability comes with complexity in sample preparation, where cell number, purity of cells and buffer are crucial for high-quality data. MARS Pre-CyTOF buffer together with MARS SP acoustic wash support CyTOF users in gentle, efficient sample preparation.

1. Astle, John M., and Huiya Huang, Frontiers in Oncology 11 (2021), https://doi.org/10.3389/fonc.2021.704464

2. CyTOF® is a trademark (™) and/or registered trademark (®) of Fluidigm Corporation or its affiliates in the United States and/or other countries.

Acoustic wash for simplified sample preparation

Acoustic debris removal is a label-free method that relies on acoustic waves to separate cells based on their physical properties, such as size and density.
Acoustic debris removal offers ease of operation, gentle  sample processing and high purity of separated cells. It is therefore useful for handling  fragile cell types. 

MARS Acoustic debris removal technology overview

MARS SP

The MARS SP workflow offers gentle sample handling without the need for centrifugation. It ensures high recovery and viability of enriched cells while minimizing the use of reagents and hands-on time.

Label-free white blood cell separation from RBC and debris based on the difference in physical parameters ensures gentle cell treatment.
 

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