The success of CAR T cell therapy in blood cancers highlights the need to optimize manufacturing for wider access, and efficient T cell isolation is crucial, impacting therapy effectiveness. Demand for better isolation methods is rising. CD3/CD28 microbeads are gaining attention for simplifying processes and enhancing workflow. They activate a wide range of T cells, promoting diverse responses. Our innovative approach uses Applied Cells IngenuityTM 4.5 μm beads, integrated into our automated MARS® Bar system, offering streamlined isolation with exceptional performance.