Neutrophils are very sensitive to vigorous mixing and pipetting, which often result in cell activation. Neutrophil isolation from whole blood is usually performed with RBC lysis or density gradient separation method, typically using Percoll-based or Ficoll – based protocols.
MARS CS workflow for gentle Neutrophil selection provides high purity and yield with:
- over 87% Neutrophil purity with gentle RBC lysis and our label-free Acoustic Isolation
- over 99% purity using No Lyse, No Wash Magnetic Selection
- no Ficoll gradient required